KEY PUBLICATIONS (SINCE 2007)
Li Dai, Digvijay Singh, Suoang Lu, Vishal Kottadiel, Reza Vafabakhsh, Marthandan Mahalingam, Yann R. Chemla, Taekjip Ha, Venigalla B. Rao (2021) A viral genome packaging ring-ATPase is a flexibly coordinated pentamer. Nature Communications 12:6548 https://doi.org/10.1038/s41467-021-26800-z
Zhu, Neeti Ananthaswamy, Swati Jain, Himanshu Batra, Wei-Chun Tang, Douglass A. Lewry, Michael L. Richards, Sunil A. David, Paul B. Kilgore, Jian Sha, Aleksandra Drelich, Chien-Te K. Tseng, Ashok K. Chopra, Venigalla B. Rao (2021) A Universal Bacteriophage T4 Nanoparticle Platform to Design Multiplex SARS-CoV-2 Vaccine Candidates by CRISPR Engineering. Science Advances 7(37), eabh1547. https://doi.org/10.1126/sciadv.abh1547
Rao, V. B., Fokine, A. and Fang, Q. (2021) The Remarkable Viral Portal Vertex: a plausible model for mechanism. Curr. Opin. Virol. 52:65-73. https://doi.org/10.1016/j.coviro.2021.09.004
Xiaorong Wu, Jingen Zhu, Pan Tao, Venigalla B. Rao, (2021) Bacteriophage T4 Escapes CRISPR Attack by Mini-homology Recombination and Repair. mBio 12:e01361-21. https://doi.org/10.1128/mBio.01361-21
Mo Y, Keller N, delToro D, Ananthaswamy N, Harvey SC, Rao VB, Smith DE. (2020) Function of a viral genome packaging motor from bacteriophage T4 is insensitive to DNA sequence. Nucleic Acids Res. 48(20):11602-11614. https://doi.org/10.1093/nar/gkaa875
Tao, P., Wu, X. and Rao, V.B. (2018) Unexpected evolutionary benefit to phages imparted by bacterial CRISPR-Cas9. Science Advances Vol. 4, no. 2, eaar4134. https://doi.org/10.1126/sciadv.aar4134
Tao P, Wu X, Tang WC, Zhu J, Rao V. (2017) Engineering of Bacteriophage T4 Genome Using CRISPR-Cas9. ACS Synth Biol. 2017 6(10):1952-1961. https://doi.org/10.1021/acssynbio.7b00179
Fang Q, Tang WC, Tao P, Mahalingam M, Fokine A, Rossmann MG, Rao VB. (2020). Structural morphing in a symmetry-mismatched viral vertex. Nature Communications 2020 11(1):1713. https://doi.org/10.1038/s41467-020-15575-4
Zhu, J., Tao, P., Mahalingam, M., Sha, J., Kilgore, P., Chopra, A. K. and Rao, V. (2019) A prokaryotic eukaryotic hybrid viral vector for delivery of large cargos of genes and proteins into human cells. Science Advances 5, eaax0064. https://doi.org/10.1126/sciadv.aax0064
Tao, P., Zhu, J., Mahalingam, M., Batra, H. and Rao, V.B. (2019) Bacteriophage T4 nanoparticles for vaccine delivery against infectious diseases. Advances in Drug Delivery Reviews 145:57-72. https://doi.org/10.1016/j.addr.2018.06.025
Tao P, Mahalingam M, Zhu J, Moayeri M, Sha J, Lawrence WS, Leppla SH, Chopra AK, Rao VB. (2018) A Bacteriophage T4 Nanoparticle-Based Dual Vaccine against Anthrax and Plague. mBio 9(5): e01926-18. https://doi.org/10.1128/mBio.01926-18
Islam MZ, Fokine A, Mahalingam M, Zhang Z, Garcia-Doval C, van Raaij MJ, Rossmann MG, Rao VB. (2019) Molecular anatomy of the receptor binding module of a bacteriophage long tail fiber. PLoS Pathogens 15(12):e1008193. https://doi.org/10.1371/journal.ppat.1008193
Ananthaswamy, N., Fang, Q., AlSalmi,W., Jain, S., Chen, Z., Klose, T., Sun, Y., Liu, Y., Mahalingam, M., Chand, S., Tovanabutra, S., Robb, M., Rossmann, M.and Rao, V. B. (2019) A sequestered fusion peptide in the structure of an HIV-1 transmitted founder envelope trimer. Nature Communications 10(1):873. https://doi.org/10.1038/s41467-019-08825-7
Ordyan, M., Alam, I., Mahalingam, M., Rao, V. B. and Smith, D. E. (2018) Nucleotide-dependent DNA gripping and an end-clamp mechanism regulate the bacteriophage T4 viral packaging motor. Nature Communications 9(1):5434. https://doi.org/10.1038/s41467-018-07834-2
Lin, S., Alam, T.I., Kottadiel, V., VanGessel, C.J., Tang, W-C., Chemla, Y.R. and Rao, V.B. (2017) Altering the speed of a DNA packaging motor from bacteriophage T4. Nucleic Acids Research 45(19):11437-11448. https://doi.org/10.1093/nar/gkx809
Chen Z, Sun L, Zhang Z, Fokine A, Padilla-Sanchez V, Hanein D, Jiang W, Rossmann MG, Rao VB. (2017) Cryo-EM structure of the bacteriophage T4 isometric head at 3.3-Å resolution and its relevance to the assembly of icosahedral viruses. Proc Natl Acad Sci U S A 114:E8184-8193. https://doi.org/10.1073/pnas.1708483114
Rao, V.B. and Feiss, M. (2015) Mechanisms of DNA Packaging in Large Double Stranded DNA Viruses. Ann. Rev. Virology 2(1):351-78. https://doi.org/10.1146/annurev-virology-100114-055212
Sun, L., Zhang, X., Gao S., Rao, P.A., Padilla-Sanchez V., Chen, Z., Sun, S., Xiang, Y., Subramaniam S., Rao, V.B., Rossmann, M.G. (2015) Cryo-EM structure of the bacteriophage T4 portal protein assembly at near-atomic resolution. Nature Communications 6:7548. https://doi.org/10.1038/ncomms8548
Vafabakhsh, R., Kondabagil, K., Earnest, T. M., Lee, K. S., Zhang, Z., Dai, L., Dahmen, K. A., Rao, V. B. and Ha, T. (2014) Single-molecule packaging initiation in real time by a viral DNA packaging machine from bacteriophage T4. Proc. Natl. Acad. Sci. USA 111(42):15096-15101. https://doi.org/10.1073/pnas.1407235111
Migliori, A. D., Kellera, N., Alam, T. I., Mahalingam, M., Rao, V. B., Arya, G., and Smith, D. E. (2014) Evidence for an electrostatic mechanism of force generation by the bacteriophage T4 DNA packaging motor. Nature Communications 5: 4173. https://doi.org/10.1038/ncomms5173
Tao, P., Mahalingham, M., Kirtley, M., van Lier, C. J., Sha, J., Yeager, L. A., Chopra, A. K. and Rao, V. B. (2013). Mutated and bacteriophage T4 nanoparticle arrayed F1-V immunogens from Yersenia pestis as next generation plague vaccines. PLOS Pathogens 9(7): e1003495. https://doi.org/10.1371/journal.ppat.1003495
Tao, P., Mahalingham, M., Marasa, B., Chopra, A. and Rao, V. B. (2013). In vitro and in vivo delivery of genes and proteins using the bacteriophage T4 DNA packaging machine. Proc. Natl. Acad. Sci. USA 110:5846-5851. https://doi.org/10.1073/pnas.1300867110
Kottadiel VI, Rao VB, Chemla YR. (2012) The dynamic pause-unpackaging state, an off-translocation recovery state of a DNA packaging motor from bacteriophage T4. Proc. Natl. Acad. Sci. USA 109:20000-20005. https://doi.org/10.1073/pnas.1209214109
Black, L.W. and Rao, V.B. (2012) Structure, Assembly, and DNA packaging of bacteriophage T4 head. Adv. Virus Res. 82:119-153. https://doi.org/10.1016/B978-0-12-394621-8.00018-2
Sun S, Gao S, Kondabagil K, Xiang Y, Rossmann MG, Rao VB. (2012) Structure and function of the small terminase component of the DNA packaging machine in T4-like bacteriophages. Proc Natl Acad Sci U S A. 109(3):817-22. https://doi.org/10.1073/pnas.1110224109
Kondabagil, K., Draper, B., Rao, V.B. (2012). Adenine recognition is a key checkpoint in the energy release mechanism of phage T4 DNA packaging motor. J. Mol. Biol. 415(2):329-42. https://doi.org/10.1016/j.jmb.2011.11.013
Zhang, Z., Kottadiel, V., Vafabakhsh, R., Li, Dai, Chemla,Y.R., Ha, T.J. and Rao, V.B. (2011) A promiscuous DNA packaging machine from bacteriophage T4. PLOS Biology 9:e1000592, 1-11. https://doi.org/10.1371/journal.pbio.1000592
Rao, M., Peachman, K., Li, Q., Matyas, G., Shivachandra, S., Borschel, R., Morthole, V.I., Fernandez-Prada, C. R., Alving, C. and Rao, V.B. (2011) Highly effective generic adjuvant systems for orphan or poverty-related vaccines. Vaccine 29:873-877. https://doi.org/10.1016/j.vaccine.2010.11.049
Sun, S., Rao, V.B. and Rossmann, M. Genome packaging in viruses. (2010) Curr. Opin. Struct. Biol. 20 (1):114-120. https://doi.org/10.1016/j.sbi.2009.12.006
Rao, V. B. (2009) A virus DNA gate: zipping and unzipping the packed viral genome. Proc. Natl. Acad. Sci. USA 106: 8403-8404. https://doi.org/10.1073/pnas.0903670106
Sun, S, Kondabagil, K., Draper, B., Alam, I.T., Baumann, V., Zhang, Z., Hegde., Fokine, A., Rossmann, M.G., and Rao, V.B. (2008) The atomic structure of bacteriophage T4 DNA packaging motor suggests a mechanism dependent on electrostatic forces. Cell 135:1251-1262. This paper was featured as a "LEADING EDGE" article; Williams, R.S., Williams, G.J. and Tainer, J.A. Cell 135:1169-71. https://doi.org/10.1016/j.cell.2008.11.015
Rao, V.B. and Feiss, M. The bacteriophage DNA packaging motor. (2008). Ann. Rev. Genetics 42:642-681. https://doi.org/10.1146/annurev.genet.42.110807.091545
Fuller, D. N., Raymer, D. M., Kottadiel, V. I., Rao, V. B. and Smith, D. E. (2007). Single Phage T4 DNA packaging motors exhibit large force generation, high velocity, and dynamic variability. Proc. Natl. Acad. Sci. U.S.A. 104:16868-16873. https://doi.org/10.1073/pnas.0704008104
Sun S, Kondabagil K, Gentz PM, Rossmann MG, and Rao V. B. (2007). The Structure of the ATPase that Powers DNA Packaging into Bacteriophage T4 Procapsids. Mol. Cell. 25(6):943-949. https://doi.org/10.1016/j.molcel.2007.02.013
